Expression and protein levels of ethylene receptors, CTRs and EIN2 during tomato fruit ripening as affected by 1- MCP

Many studies have focused on the plant hormone ethylene because of its key role in controlling, among others, climacteric fruit ripening and senescence. These processes can be controlled by applying 1-MCP, which tightly binds to receptors thereby blocking signaling pathway. 1-MCP is known inhibit action delay tomato fruit. Less about long term effect when inhibitory inhibition eventually released. Our objective was study this transient during terms quality, production, respiration rate expression protein abundance receptors, CTRs EIN2. For identification quantification proteins, we used an LC–MS based targeted method Parallel Reaction Monitoring (PRM), while gene done using real time qPCR. Different color stages tomatoes were harvested treated with subsequently stored follow up postharvest ripening. The difference previous that sampled at different physiological (and not time), matching untreated control This allows properly compare underlying regulation pathway a 1-MCP-mediated suppression We hypothesized levels components would for due reduced ethylene-mediated autocatalytic feed-back. results showed mature green stage lower subsequent as compared fruit, suggesting effectively inhibited 1-MCP. However, these higher production also equal As are negative regulators signaling, decreasing new proteins could activate downstream genes. lead levels, turn compensate mediated